Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 6;10:2484. doi: 10.1038/s41467-019-10127-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — PRL3 is expressed on the surface of exosomes from PRL3⁺ cancer cells. a Representative PRL3 western blot (WB) in purified exosomes from MHCC-LM3 and Hep53.4 liver cancer cells cultured for 24 h in serum-free media. Paxillin served as a negative marker for exosomes, whereas TSG101 is a positive exosomal marker. b Immunofluorescence analysis of MHCC-LM3 cells transiently transfected with green fluorescence protein (GFP) or GFP-PRL3 to reveal plasma membrane enrichment of PRL3. Scale bar, 20 µm. c Representative PRL3 WB in purified exosomes from cells in b after culturing for 24 h in serum-free media. Calnexin served as a negative marker for exosomes. d Purified exosomes from Hep53.4 and Hep53.4-PRL3 were analyzed as in c. e Purified exosomes from cells in c, d were assayed for sensitivity of exosomal PRL3 to proteinase K (Prot. K) digestion in the absence or presence of 1% Triton X-100 detergent. TG101 and Alix are intravesicular exosomal proteins. f Proposed model of the localization of PRL3, TSG101, and Alix in exosomes, based on Prot. K susceptibility observed in e. Source data are provided as a Source Data file