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. 2019 May 31;10:703. doi: 10.3389/fpls.2019.00703

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Copyright © 2019 Liu, Sun, Xue, Mao, Jia and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Deduced GhSAD expression patterns of GhA-SAD5 (A), GhD-SAD5 (B), GhA-SAD6 (C), GhA-SAD7 (D), GhD-SAD7 (E), and GhD-SAD8 (F) in various cotton tissues. Bar charts show the relative expression levels of GhSAD genes normalized to that of Histone3 and EF-1α measured by qRT-PCR. The analysis was performed with three biological samples for each tissue. Ovu-5d, 10d and 20d represent 5, 10, and 20 DAF of developing cottonseed ovules. The method of 2^-ΔΔcq was used in this analysis. “^∗” represents statistically significant difference values of P < 0.05 and “^∗∗” for P < 0.01 based on two-tailed Student’s t-tests.