Table 1.
Comparison of the eight amino acid residues comprising the region of the catalytic sites of GhSADs.
| Ricinus communis | Arabidopsis thaliana | Gossypium hirsutum | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| RcSAD1 | AtFAB2 | GhA-SAD1 | GhA-SAD2 | GhA-SAD3 | GhA-SAD4 | GhA-SAD8 | GhA-SAD9 | GhD-SAD1 | GhD-SAD2 | GhD-SAD3 | GhD-SAD6 |
|
Group 1:ω-9 producing isoforms | |||||||||||
| L118 | L156 | L141 | L150 | L140 | L151 | L150 | L150 | L150 | L141 | L151 | L150 |
| F189 | F227 | F212 | F211 | F211 | F212 | F221 | F221 | F221 | F212 | F222 | F221 |
| L115 | L153 | I138 | L147 | I137 | L148 | L147 | L147 | L147 | I138 | L149 | L147 |
| T117 | T155 | T140 | T149 | T139 | T150 | T149 | T149 | T149 | T140 | T150 | T149 |
| G188 | G226 | G211 | G210 | G210 | G211 | G220 | G220 | G220 | G211 | G221 | G220 |
| T181 | T219 | T204 | T213 | T203 | T214 | T213 | T213 | T213 | T204 | T214 | T213 |
| P179 | P217 | P202 | P211 | P201 | P212 | P211 | P211 | P211 | P202 | P212 | P211 |
| M114 | M152 | M137 | M146 | M136 | M147 | M146 | M146 | M146 | M137 | M148 | M146 |
|
Arabidopsis thaliana |
Dauricum unguis-cati |
Gossypium hirsutum |
|||||||||
| AtAAD3 | AtAAD2 | Muc-PAD | GhD-SAD4 | GhA-SAD5 | GhD-SAD5 | GhA-SAD6 | GhA-SAD7 | GhD-SAD7 | GhD-SAD8 | ||
|
ω-7 producing isoforms |
Group 2: ω-7 producing isoforms? |
||||||||||
| L153 | L163 | Y1W152 | L151 | L31 | L150 | L152 | P1M148 | P1M148 | L151 | ||
| F224 | F234 | F223 | F222 | F102 | F221 | F223 | P1A219 | P1 A219 | P1Y222 | ||
| L150 | L160 | L149 | L149 | L28 | L147 | L149 | L145 | L145 | L148 | ||
| T152 | T162 | T151 | T150 | P1R30 | P1R149 | T151 | T147 | T147 | T150 | ||
| G223 | G233 | G222 | G221 | P1T101 | P1T220 | P1T222 | G218 | G218 | P1A221 | ||
| Y1F216 | Y1F226 | T215 | T214 | P1I94 | P1I213 | P1A215 | P1S211 | P1S211 | P1A214 | ||
| Y1S214 | Y1S224 | P213 | P212 | P1V192 | P1V211 | P1P213 | P1S209 | P1S209 | P1M212 | ||
| Y1T149 | Y1T159 | M148 | P1I148 | P1I27 | M146 | M148 | P1L144 | P1L144 | M147 | ||
Sequences were conducted by GeneDoc software using RcSAD1, AtFAB2, AtAAD3 as well as Muc-PAD as temples. The GenBank or TAIR accession numbers of the temple sequences listed as the follows: RcSAD1 (NP_001310659.1) from R. communis; AtFAB2 (At2g43710), AtAAD2 (At3g02610), and AtAAD3 (At5g16230) from A. thaliana; Muc-PAD (AFV61670.1) from D. unguis-cati. Orange and pink color represent varied amino acids residues in the domain of 16:0-ACP specific isoforms and the putative 16:0-ACP specific GhSADs, respectively, compared to the 18:0-ACP specific isoforms characterized.