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. 2019 May 31;10:1030. doi: 10.3389/fimmu.2019.01030

Figure 4.

Figure 4

C5 Convertase binding. Autoantibody binding to the in vitro assembled C3 convertase was evaluated. In this case, group 1 and also group 2 antibodies bind to the assembled C5-convertase (**p < 0.01). IgGs prepared from NHS show background binding (stippled line). In addition, more group 1 antibodies bind to the C5-convertase as compared to group 2 antibodies (**p < 0.01). The C5-convertase was assembled on an ELISA plate by immobilizing C3b (10 μg/ml) and adding Factor B (1 μg/ml), Factor D (0.5 μg/ml), properdin (1 μg/ml), and C3 (10 μg/ml).