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. 2019 May 31;10:1030. doi: 10.3389/fimmu.2019.01030

Figure 5.

Figure 5

Autoantibodies modulate C5a generation. (A) Representative patient's autoantibodies from group 1 (#A and #J) and group 2 (#a and #r) increase C5a generation significantly when compared to IgGs prepared from NHS compared to control (**p < 0.01, ***p < 0.001). (B) IgGs from all patients except # 607 (group 1), # 328, #2367 (both group 2), enhance C5a generation in a significant manner (*p < 0.05, **p < 0.01, ***p < 0.001). Purified IgGs from each patient was added to complement active NHS at 37°C and the mixture was incubated for 45 min. C5a generation was monitored by ELISA and compared to C5a generation in NHS (stippled line).