Fig. 4. IGF-1 gene transfer to CCl₄-induced rat models reduces nuclear progerin and p53 protein levels of primary rat hepatocytes.

a The H₂O₂ content of primary rat hepatocytes, isolated from CCl₄-induced rat models on Day 6 and Day 28. *P < 0.05 versus the Vehicle group on Day 6; ^#P < 0.05 versus the CCl₄ + LV-CTR group on Day 6; ^&P < 0.05 versus the Vehicle group on Day 28; ^$P < 0.05 versus the CCl₄ + LV-CTR group on Day 28. b Representative immunoblots of progerin, Lamin A/C, and p53 of primary hepatocytes isolated from the CCl₄-induced rat models on Day 6 and Day 28. c The progerin protein expression (red) in hepatocytes of the four groups (Vehicle, CCl₄, CCl₄ + LV-CTR, CCl₄ + LV-IGF-1) on Day 6 and Day 28, shown by immunofluorescence. Nuclear were showed by DAPI (blue). Scale bar: 50 μm. n = 6 per group