Fig. 5. H₂O₂ induces hepatocyte premature senescence via the p53/progerin-dependent pathway.

Freshly hepatocytes, isolated from normal rats and cultured in vitro, were transfected with p53 siRNA, progerin siRNA, or nontarget siRNA (called NC), and then administered with H₂O₂ (200 nM) for 24 h. a SA-β-gal activity in primary rat hepatocytes of the four groups (NC, NC + CCl₄, CCl₄ + si p53, si p53), was revealed by SA-β-gal staining. Scale bar: 50 μm. SA-β-gal positive cells are quantified in the graph, right. *P < 0.05 versus the NC group; ^#P < 0.05 versus the NC + H₂O₂ group. b Representative immunoblots of p53, p21, progerin, and Lamin A/C of primary rat hepatocytes of the six groups (CTR, H₂O₂, NC, NC + CCl₄, CCl₄ + si p53, and si p53). The relative protein expression is quantified in the graph, right. ^&P < 0.05 versus the CTR group; ^*P < 0.05 versus the NC group; ^#P < 0.05 versus the NC + H₂O₂ group. c SA-β-gal activity in primary rat hepatocytes of the four groups (NC, NC + CCl₄, CCl₄ + si progerin, and si progerin), was revealed by SA-β-gal staining. Scale bar: 50 μm. SA-β-gal positive cells are quantified in the graph, right. *P < 0.05 versus the NC group; ^#P < 0.05 versus the NC + H₂O₂ group. d Representative immunoblots of progerin and Lamin A/C of primary rat hepatocytes of the six groups (CTR, H₂O₂, NC, NC + CCl₄, CCl₄ + si progerin, and si progerin). The relative protein expression is quantified in the graph, right. ^&P < 0.05 versus the CTR group; *P < 0.05 versus the NC group; ^#P < 0.05 versus the NC + H₂O₂ group