Table 3.
Antibody pairs tested in plasma and with recombinant proteins.
| Samples | Detection Antibodies | Capture Antibodies | ||||
|---|---|---|---|---|---|---|
| anti-IGFBP2 | anti-IGF2 | anti-BCHE | anti-IGF1 | anti-DERA | ||
| Plasma | anti-IGFBP2 | + | + | + | − | − |
| anti-IGF2 | + | + | − | − | − | |
| anti-BCHE | − | − | + | − | − | |
| anti-IGF1 | − | − | − | + | − | |
| anti-DERA | − | − | − | − | − | |
| Rec-IGFBP2 | anti-IGFBP2 | + | − | − | − | − |
| Rec-IGF2 | anti-IGF2 | − | − | − | − | − |
| Rec-BCHE | anti-BCHE | − | − | + | − | − |
| Rec-IGF1 | anti-IGF1 | − | − | − | − | − |
Annotation (+): Trends from sample dilution assays were obtained with at least one combination of antibodies for the same protein either in heat-treated or untreated samples. Annotation (−): No sample concentration dependent data was obtained. (Details regarding each single pair are listed in Supplementary Excel Sheet “Reagents_Lot_Numbers”. Catalog numbers of the functional pairs are indicated in Fig. 4 and S-Fig. 9).