Skip to main content
. 2019 May 31;12:144. doi: 10.3389/fnmol.2019.00144

Figure 2.

Figure 2

Kv4.2 channels are SUMOylated in a heterologous expression system. A mouse Kv4.2 channel with a C-terminal GFP tag (Kv4.2g) was stably expressed in Human Embryonic Kidney (HEK) cells (HEK-Kv4.2g). IP experiments were performed on cell lysates from HEK-Kv4.2g cells or HEK parental cells using an anti-GFP or a negative control IgG antibody. Western blots containing IP product were probed using an (A) anti-GFP, (B) anti-Kv4.2, or (C) anti-SUMO-2/3 antibody. Experiments were repeated three times. The Kv4.2-GFP protein was detected as a 100 kD band (asterisk) using antibodies against GFP, Kv4.2, and SUMO-2/3. This band was never observed when a negative control IgG or HEK parental cells were used in the IP. A 70 kD band was also detected by all antibodies (arrow head), and it most likely represents cleavage of the channel. This signal was not included in subsequent quantifications. This most likely represents sample degradation. The 50 kD band observed in all lanes (double asterisk) likely represents the IP antibodies.