Table 1.
Primary antibodies.
| Antigen | Verified by | Species, manufacturer and catalog number | Concentration used |
|---|---|---|---|
| Kv4.2 | Specificity verified by the company. WB analysis of rat membranes probed with anti-Kv4.2 (1:200) revealed a 68 kD band. | rabbit, alomone labs, #APC-023 | IP: 8.3 μL antibody per 1,000 μg protein; WB: 1:750 |
| GFP (IP) | Specificity verified by the company. Ab detects 5 ng recombinant GFP expressed in HEK293 cells. | rabbit, abcam, ab290 | IP: 1 μL antibody per 500 μg protein |
| GFP (WB) | Specificity verified by the company. WB analysis of untransfected COS and COS cells transfected with pCruz GFP-Lac Z | rabbit, Santa Cruz Biotechnology, sc-8334 | WB: 1:3,000 |
| SUMO-1 | Specificity verified by the company. WB analysis of HEK293 expressing SUMO-1. | rabbit, Santa Cruz Biotechnology, sc-9060 | WB: 1:1,000 |
| SUMO-2/3 | Specificity verified by the company. WB analysis of HEK293 cells transfected with SUMO-2 | rabbit, Santa Cruz Biotechnology, sc-32873 | WB (HEK cells): 1:3,000; WB (rat brain): 1:1,000 |
| SUMO-2/3 | Specificity verified by the company. Ab recognizes 15 and 18 kD bands in HeLa extract on WB. | rabbit, abcam, ab3742 | WB (HEK cells): 1:1,000; WB (rat brain): 1:750 |
| Na+/K+-ATPase | Specificity verified by the company. Positive signal on WB using HEK293 whole cell lysate. | mouse, abcam, ab7671 | WB: 1:3,000 |
| Actin | Specificity verified by the company. WB analysis of C32 whole cell lysate | rabbit, Santa Cruz Biotechnology, sc-1616-R | WB: 1:2,000 |
| BSA | Specificity verified by the company. BSA was loaded onto a gel. WB analysis revealed a 68 kD band when probed with anti-BSA antibody. | rabbit, ThermoFisher, A11133 | WB: 1:20,000 |