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. 2019 May 31;13:241. doi: 10.3389/fncel.2019.00241

FIGURE 4.

FIGURE 4

Morphological changes in microglia in the cerebellum after irradiation. (A) Microglia in the cerebellum were immunostained by Iba-1 and classified into ramified, hyper-ramified, bushy, and amoeboid phenotypes based on their morphological characteristics. (B) The number of Iba-1–labeled cells in the cerebellum decreased significantly at 5 days after irradiation. (C) Iba-1–labeled cells with different morphologies were counted separately in the cerebellar IGL. (D) Representative pictures showing the Iba-1–labeled cells in the cerebellar white matter. (E) Iba-1–labeled cells with different morphologies were counted separately in white matter of the cerebellum. (F) Iba-1–labeled cells with different morphologies were counted separately in the cerebellar ML. (G) The protein levels of IL-1β, IL-2, IL-4, IL-6, IL-10, KC, and CCL2 in the cerebellum were detected by Luminex assay after irradiation in the Atg7 KO and WT pups. n = 7/group for the Iba-1 staining and Luminex assay. ∗∗∗p < 0.001.