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. 2019 May 3;116(11):2121–2130. doi: 10.1016/j.bpj.2019.04.033

Figure 5.

Figure 5

SAXS data for UNC-6 ΔC in low- and high-ionic strength buffer (magenta and light blue, respectively). (A) Light scattering profile of UNC-6 ΔC after passing through the Superdex 200 column. (B) Raw scattering data from the protein peak chosen at 250–300 frames for UNC-6 ΔC in the low-ionic strength buffer and 300–350 frames for UNC-6 ΔC in high-ionic strength. Inset to (B) displays the DAMMIN fit to the raw scattering data in high-ionic strength buffer (C) Guinier plots show that the scattering data fit to a line. (D) Pair-distance distribution P(r) plot of averaged SAXS data suggest that UNC-6 ΔC has a defined shape with an extended tail that reaches to 14.3 nm in high-ionic strength buffer and 37 nm in low-ionic strength buffer. To see this figure in color, go online.