Fig. 1.

Results of size and concentration measurements of purified uEVs using nanoparticle tracking analysis (NTA) in scattering mode (SM, black) and in fluorescence mode (FM, blue): spin column chromatography (A), immunoaffinity (B), precipitation (C), membrane affinity (D) and ultracentrifugation combined with density gradient (E). (n = 6; p value, adjusted for multiple comparisons: * < 0.05, ** < 0.01) (a) Illustration of the mean (box-whisker-plot: whiskers indicating minimum and maximum values) and modal (dot-plot: mean ± SD) particle diameter in nanometer (nm). (b) Detected particle concentration corrected for sample dilution and normalized to urinary creatinine (U_Crea); left and right Y axes log10 transformed (mean ± SD).