Fig. 4.

Assignment of small RNA sequencing results to different RNA species (n = 6): spin column chromatography (A), immunoaffinity (B), precipitation (C), membrane affinity (D) and ultracentrifugation combined with density gradient (E). (a) Mean relative frequency of reads that mapped to microRNA (miRNA), transfer RNA (tRNA), small nucleolar RNA (snoRNA), small nuclear RNA (snRNA), ribosomal RNA (rRNA), remained unmapped, were shorter than 16 nucleotides (short) or did not show any adapter). (b) Overlap of distinct miRNA species detected in sequencing libraries from each isolation method, data are filtered for miRNAs with more than 20 reads per group.