Table 1.

Overview of applied strategies to purify small extracellular vesicles from urine.

Purification type:	Based on:	Manufacturer:
A	Spin column chromatography	Urine Exosome Purification and RNA Isolation Midi Kit (Norgen Biotek, Norway)
B	Immunoaffinity	Exosome Isolation Kit Pan, human (Miltenyi Biotec, Germany)
C	Membrane affinity	exoRNeasy Serum/Plasma Midi Kit (Qiagen, Germany)
D	Precipitation	miRCURY Exosome Isolation Kit – Cells, urine and CSF (Exiqon, Denmark)
E	Ultracentrifugation with density gradient	Optima LE-80 K (Beckman Coulter, USA) and OptiPrep (Merck, Germany)