Table 2.
Performance indicators for RNA extraction and small RNA-Seq.
| A | B | C | D | E | |
|---|---|---|---|---|---|
| Total RNA [pg/ml urine] | |||||
| MEAN | 289.5 | 277.0 | 457.0 | 542.0 | 331.0 |
| SD | 134.0 | 90.9 | 216.8 | 292.9 | 233.0 |
| Total RNA [ng/g UCrea] | |||||
| MEAN | 456.3 | 450.7 | 697.5 | 852.6 | 523.0 |
| SD | 86.3 | 72.6 | 162.2 | 289.7 | 281.3 |
| Total readcounts [million reads] | |||||
| MEAN | 9.01 | 10.8 | 13.6 | 14.1 | 11.0 |
| SD | 1.79 | 2.90 | 2.80 | 3.81 | 1.49 |
| miRNA mapped readcounts [%] | |||||
| MEAN | 2.9 | 5.0 | 3.4 | 5.8 | 3.7 |
| SD | 0.6 | 2.2 | 1.1 | 2.3 | 0.7 |
| Individually detected canonical miRNAs | |||||
| Absolute number | 102 | 119 | 114 | 119 | 124 |
Spin column chromatography (A), immunoaffinity (B), precipitation (C), membrane affinity (D) and ultracentrifugation combined with density gradient (E).