Table 3.
Top 10 expressed miRNAs identified by small RNA sequencing in every uEV isolation method.
| MEAN [reads] | A [%] | B [%] | C [%] | D [%] | E [%] | |
|---|---|---|---|---|---|---|
| miR-451a | 18,970 | +110 (24) | −3 (44) | −9 (25) | −65 (21) | −32 (30) |
| miR-148a-3p | 14,326 | −91 (4) | +112 (118) | +10 (76) | +11 (29) | −42 (25) |
| miR-486-5p | 12,702 | +110 (28) | −4 (46) | −10 (27) | −64 (22) | −31 (32) |
| miR-26a-5p | 6,547 | −82 (4) | −13 (32) | +2 (52) | +51 (27) | +42 (66) |
| miR-92a-3p | 6,021 | +100 (27) | −3 (37) | −13 (22) | −57 (19) | −28 (26) |
| miR-30d-5p | 5,251 | −78 (2) | +28 (55) | −4 (23) | +54 (36) | 0 (31) |
| let-7a-5p | 3,947 | −79 (4) | +8 (47) | +1 (30) | +49 (32) | +21 (61) |
| miR-191-5p | 3,011 | +95 (24) | −6 (40) | −11 (18) | −56 (16) | −21 (24) |
| let-7i-5p | 2,802 | −9 (10) | −9 (15) | −9 (23) | +4 (14) | +23 (33) |
| miR-30c-5p | 2,127 | −66 (2) | +4 (32) | +30 (45) | +28 (21) | +4 (43) |
Spin column chromatography (A), immunoaffinity (B), precipitation (C), membrane affinity (D) and ultracentrifugation combined with density gradient (E). miRNAs are ranked by their mean normalized reads over all groups. Method-specific variances in miRNA detection are indicated by their percentage deviations from the average miRNA-specific reads with standard deviations in brackets.