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Gel Filtration of Recombinant Galectin-1 and Variants. Galectin-1 preparations purified by metal chelation chromatography were subjected to gel filtration on a Sephadex G-50 gel filtration column (60 × 1 cm; equilibrated in 30 mM Tris-HCl buffer pH 7.5). Fractions of 2 ml were collected, and absorbances at 280 nm measured. ▴ Normal galectin-1 ▪ N46 D mutant 
P79R mutant 
Bacterial lysozyme.
