Figure 1.

TPM4 was not involved in cell proliferation. (A) TPM4 was extensively expressed in lung cancer cells. The level of TPM4 in lung cancer cells was determined by western blot. GAPDH is used as a loading control. (B) TPM4 knockout cell model was constructed by CRISPR/CAS9. TPM4 protein level was detected by Western blot, with GADPH used as the internal loading controls. (C, D) Cells were seeded in 96-well plates and an MTS assay was performed. Absorbance at 490 nm (y axis) was measured at 24-hr intervals. The data are expressed as the mean ± standard error of the mean from three separate experiments.

Abbreviations: CRISPR, clustered, regular lyinterspaced, short palindromic repeats; MTS, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium.