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. 2019 May 31;12:4055–4063. doi: 10.2147/OTT.S198542

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© 2019 Zhao et al.

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TPM4 was involved in lung cancer cell migration. (A,B) Transwell migration assay performed. Cells migrated through the chamber was counted in five microscopic fields and standardized by H1299 group and A549 group, respectively. The data is expressed as mean ± SEM. Bars represent 50 μm. ** p<0.01 (unpaired Student's t-test). (C) Wound healing assay was performed with TPM4 knock out cell clones as well as control cells for 0–16 hrs. Bars represent 50 μm. (D) Expression of mesenchymal proteins, vimentin and β-catenin, transcription factors, snail, and ZEB1 was examined by immunoblotting. GAPDH is used as a loading control.