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. 2019 May 31;12:4055–4063. doi: 10.2147/OTT.S198542

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© 2019 Zhao et al.

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F-actin assembly was increased when TPM4 was upregulated. (A) WB technique was used to determine F-actin level in TPM4 upregulating cells as well as control cells. The actin level was used as a control, with GADPH used as the internal loading controls. (B, C) F-actin was labeled by immunofluorescence staining. The red signal represents the staining of F-actin, the green signal represents the fluorescence of GFP, and the blue signal represents the nuclear DNA staining by Hoechst 33342. Bars represent 20 μm.

Abbreviation: WB, Western blot.