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. 2019 Mar 19;27(6):1087–1100. doi: 10.1016/j.ymthe.2019.03.010

Table 1.

Primer Sequences for qRT-PCR

Gene GenBank No. Direction Primer Sequence
Human CD116 NM_001161529.1 F 5′-GACTCCAGGACGATGAAT-3′
R 5′-CACGACTCTGTTCTTCTTG-3′
Human CSF-2 NM_000758 F 5′-ATGATGGCCAGCCACTACAA-3′
R 5′-AGCAGTCAAAGGGGATGACA-3′
Human fibronectin NC_000002.12 F 5′-GAGAATGGACCTGCAAGCCCA-3′
R 5′-GTGCAAGTGATGCGTCCGC-3′
Human NANOG NM_024856 F 5′-ACATGCAACCTGAAGACGTGTG-3′
R 5′-CATGGAAACCAGAACACGTGG-3′
Human PPIA NM_021130 F 5′-TGCCATCGCCAAGGAGTAG-3′
R 5′-TGCACAGACGGTCACTCAAA-3′
Human Snail2 NC_000008.11 F 5′-TACCGCTGCTCCATTCCAGC-3′
R 5′-CATGGGGGTCTGAAAGCTTG-3′
Human SOX2 NM_003106.3 F 5′-AAATGGGAGGGGTGCAAAAGAGGAG-3′
R 5′-CAGCTGTCATTTGCTGTGGGTGATG-3′
Human twist NC_000007.14 F 5′-CCTGCGCAAGATCATCCCCA-3′
R 5′-GCTGCAGCTTGCCATCTTGGA-3′
Mouse C-MYC NM_010849 F 5′-CGCACACACAACGTCTTGGA-3′
R 5′-AGGATGTAGGCGGTGGCTTT-3′
Mouse HPRF NM_013556 F 5′-GCCTAAGATGAGCGCAAGTTG-3′
R 5′-TACTAGGCAGATGGCCACAGG-3′
Mouse KLF4 NM_010637 F 5′-GGTGCAGCTTGCAGCAGTAA-3′
R 5′-AAAGTCTAGGTCCAGGAGGT-3′
Mouse OCT4 NM_013633 F 5′-GCATTCAAACTGAGGCACCA-3′
R 5′-AGCTTCTTTCCCCATCCCA-3′
Mouse SOX2 NM_011443 F 5′-GAAGCGTGTACTTATCCTTCTTCAT-3′
R 5′-GAGTGGAAACTTTTGTCCGAGA-3′

F, forward; R, reverse.