Fig. 6.

The changes of GCaMP6 and mCherry fluorescence signals in LC and RVM/DR neurons.

Response magnitude in the GCaMP6 fluorescence was summarized for LC neurons (A, D), RVM neurons (B, E), and DR neurons (C, F) in experiment-1 (only G-CaMP experiment, A–C) and experiment-2 (both G-CaMP6 and mCherry were used, D–F). Nociceptive stimuli of tail pinch (Pinch) and high heat (55 °C) increased G-CaMP6 fluorescence in both experiments while gentle touch to the tail (Gentle) and low heat (25 °C) stimuli did not induce significant changes in fluorescence signal intensity. Fluorescence of mCherry did not change in response to nociceptive nor control stimuli (G–I). P values were calculated using paired t-test between maximum signal intensity after the stimulation and that during baseline period. * indicates p < 0.05. Values are mean ± SEM for 5 animals in experiment-1 and 3 animals in experiment-2.