Fig. 7.

The changes of the activity of LC NA neurons and RVM/DR 5-HT neurons by acute nociceptive stimuli.

Response magnitude (leftmost column) and time-related characteristics (2nd column: onset latency; 3rd column: peak latency; rightmost column: return to baseline time) in the responses of LC NA neurons (A), RVM 5-HT neurons (B), and DR 5-HT neurons (C). Gentle touch to the tail (Gentle) and low heat (25 °C) stimuli did not induce significant changes in fluorescence signal intensity. Therefore, time-related characteristics in the responses were calculated only for tail pinch and high heat (55 °C) stimuli. P values in the figures in the leftmost column were calculated using paired t-test between maximum signal intensity after the stimulation and that during baseline period. P values in the figures in the right three columns were calculated using paired t-test between pinch stimulus and high heat stimulus. * indicates p < 0.05. Values are mean ± SEM in 8 animals.