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. 2019 Apr 9;27(6):1195–1205. doi: 10.1016/j.ymthe.2019.04.002

Figure 2.

Figure 2

Expression of CoChR-GFP in TKO Mouse Retinas In Vivo

(A and B) Representative immunofluorescence images of CoChR-3M-GFP in retinal whole mounts at low (A) and high magnification (B). (C–F) Co-labeling of GFP with RBPMS, an RGC-specific marker, in retinal vertical sections (C and D) and in retinal whole mounts (B, E, and F). (G) The density of RGCs in control retinas and CoChR-treated retinas was assessed by immunolabeling for RBPMS (red). The data are presented as means ± SD (n = 4 retinas). 94%, 95%, and 92% of RGCs in wt-CoChR-, CoChR-LC-, and CoChR-3M-treated retinas were co-labeled with GFP, respectively. The densities of RGCs were not significantly different between control and treated retinas for all three CoChRs (p > 0.05; one-way ANOVA). The results were obtained from the mice 6 months after virus vector injection. Scale bars, 500 μm (A), 50 μm (C and D), and 100 μm (B, E, and F).