Table 1.
Information for primary antibodies
| Molecule | Host | Dilution | Source | Specificity information | RRID |
|---|---|---|---|---|---|
| Calbindin (CB) | Rb | 1:5000 | Swant, catalog #CB-38 (lot #5.5) | No signal in knockout. Mouse knockout: Airaksinen et al., 1997; characterization in rat hippocampus: Sloviter, 1989. | AB_2721225 |
| Calretinin (CR) | Rb | 1:500–1:1000 | Swant, catalog #7699/3H (lot #18299) | Western blot supplied by Swant. No signal in knockout animals: Schiffmann et al., 1999. | AB_10000321 |
| Calretinin (CR) | Gt | 1:1000 | Swant, catalog #CG1 | Antibody does not label the brain of CR knock-out mice (manufacturer's technical information). | AB_10000342 |
| Choline acetyltransferase (ChAT) | Gt | 1:500 | Chemicon, catalog #AB144P | Western blot (manufacturer's technical information). | AB_2079751 |
| Gephyrin | Ms | 1:500 | Synaptic Systems catalog #147 021 | Western blot; band at 93 kDa. No Geph7a signal in knockout. Co-purifies with glycine receptor. Mouse knockout: Feng et al., 1998. Antibody generation: Pfeiffer et al., 1984. | AB_2232546 |
| GFP | Ck | 1:500 | Aves Labs, catalog #GFP-1020 | Antibody does not label the brain of wild-type control mice. | AB_10000240 |
| Muscarinic acetylcholine receptor M2 (M2R) | Rt | 1:400 | Chemicon, catalog #MAB367 lot #2383976 | Distinct from other subtypes; characterized by Levey et al., 1991. | AB_94952 |
| Metabotropic glutamate receptor 1a (mGluR1a) | Gp | 1:500–1:1000 | Frontier Institute, catalog #mGluR1a-GP-Af660 | Characterized by Tanaka et al., 2000. | AB_2531897 |
| Neuronal Ca2+-binding protein 1 (NECAB 1) | Ms | 1:500 | Abnova, catalog #H00064168-B01P | Western blots; as expected, no cross-reactivity with NECABs 2 and 3. Mouse brain and antibody generation: Sugita et al., 2002. | AB_2149170 |
| Neuronal nitrogen oxide synthase (nNOS) | Rb | 1:1000 | EMD Millipore, catalog #AB5380 | Does not cross react with iNOS or eNOS. No signal in knockout for rabbit antibody (Zymed/Invitrogen) of same mass. Mouse knockout and similar rabbit antibody: Gyurko et al., 2002. | AB_91824 |
| Substance P receptor (NK1R) | Rb | 1:500 | Millipore, catalog #AB5060, lot #LV1525037 | Characterized by Shigemoto et al., 1993. | AB_2200636 |
| Parvalbumin (PV) | Rb | 1:1000 | Swant, PV-28 | No signal in knockout, shown by Swant. Similar to other parvalbumin antibodies. Mouse knockout: Schwaller et al., 1999. | AB_2315235 |
| Parvalbumin (PV) | Gt | 1:1000 | Swant, catalog #PVG-214, lot #3.6 | No signal in knockout, shown by Swant. Similar to other parvalbumin antibodies. Mouse knockout: Schwaller et al., 1999. | AB_2313848 |
| Parvalbumin (PV) | Gp | 1:5000 | Synaptic Systems, catalog #195 004, lot #5 | Western blot; abolished by pre-absorption with recombinant parvalbumin. Same labeling as Synaptic Systems rabbit antibody 195 002 and Swant monoclonal antibody 235. Rat hippocampus: Kosaka et al., 1987, Sloviter, 1989. Mouse knockout: Schwaller et al., 1999. | AB_2156476 |
| Purkinje cell protein 4 (PCP4) | Rb | 1:1000 | Santa Cruz, catalog #sc-74816, lot #G0814 | Characterized by San Antonio et al., 2014. | AB_2236566 |
| Pro-cholecystokinin (pro-CCK) | Rb | 1:500 | April 2005 gift (similar to Frontier Institute, CCK-pro-Rb-Af350) | Similar labeling to two noncommercial antibodies characterized by Sloviter and Nilaver, 1987, Morino et al., 1994. | AB_2571674 |
| Regulator of G-protein signaling 14 (RGS-14) | Ms | 1:500–1:1000 | NeuroMab, catalog #75–170, clone N133/21 | Characterized by manufacturer (see datasheet). | AB_2728538 |
| SATB1 (N-14) | Rb | 1:200 | Abcam, catalog #ab70004 | No signal in knockout for a similar rabbit antibody. Western blot, different band to SATB2 at mouse P1. Neuron specificity shown by NeuN colocalization. Original rabbit antibody: Dickinson et al., 1992. Mouse cortex: Huang et al., 2011. Mouse knockout: Balamotis et al., 2012. | AB_1270545 |
| SATB1 (N-14) | Gt | 1:200–1:250 | Santa Cruz, catalog #sc-5989 | Similar labeling to rabbit antibody ab70004. Mouse cortex: Huang et al., 2011. Mouse knockout: Balamotis et al., 2012. | AB_2184337 |
| Secretagogin | Gt | 1:1000 | R&D Systems, catalog #AF4878 lot #CASX0115091 | Characterized by Mulder et al., 2009. | AB_2269934 |
| SMI32 (neurofilament H non-phosphorylated) | Ms | 1:1000 | Covance, catalog #SMI-32R lot #14835102 | Similar to immunoreactivity characterized in primates by Campbell and Morrison, 1989, and in rats by Ouda et al., 2012. | AB_509997 |
| Somatostatin (SOM) | Ms | 1:500 | GeneTex, catalog #gtx71935 clone SOM-018 | Same labeling as a rat antibody Chemicon MAB354. No signal in preabsorption test for rat antibody. Mouse hippocampus, rat antibody: Jinno and Kosaka, 2000. Rat antibody test: Kubota et al., 2011. | AB_383280 |
| Somatostatin (SOM) | Rb | 1:50 | GenWay Biotech, catalog #18-783-76392 | Similar distribution and subcellular localization as Ms anti-SOM. | AB_1027453 |
| Vesicular acetylcholine transporter (VAChT) | Gt | 1:1000 | Millipore catalog #ABN100 | This antibody recognizes the C-terminus of vesicular acetylcholine transporter (VAChT). | AB_2630394 |
| Vesicular GABA transporter (VGAT) | Gp | 1:500 | Synaptic Systems, catalog #131 004 | Similar to C- and N-terminal fusion protein antibodies. Former gave additional unknown lower mass band in brain extract but same immunoreactivity as specific latter antibody; see rat cortex: Chaudhry et al., 1998. Similar rabbit antibody generation: Takamori et al., 2000. | AB_887873 |
| Vesicular glutamate transporter 2 (VGLUT2) | Gp | 1:2000 | Synaptic Systems, catalog #135 404, lot #135404/16 | Similar to immunoreactivity characterized in mouse hippocampus by Herzog et al., 2006. | AB_887884 |
Rb, Rabbit; Gt, goat; Ms, mouse; Ck, chicken; Rt, rat; Gp, guinea pig.