Table 1.
Anti-HCAR1 primary antibody specificity
Anti-HCAR1 primary antibody (source, reference) | Epitope | IHC |
WB |
||
---|---|---|---|---|---|
WT | HCAR1 KO | WT | HCAR1 KO | ||
Santa Cruz Biotechnology, SC-32647 | C-terminal, extracellular domain of human HCAR1 | § | § | + | + |
Santa Cruz Biotechnology, SC-32648 | C-terminal, cytoplasmic domain of human HCAR1 | + | + | − | − |
Sigma-Aldrich, SAB1300090 | Mouse GPR81-S296, aa 276–329 | + | + | + | + |
Sigma-Aldrich, SAB1300793 | Rat GPR81-R320, aa 286–332 | + | + | § | § |
Sigma-Aldrich, SAB1300089 | Human GPR81–296, aa 310–353 | + | + | + | + |
Sigma-Aldrich, SAB1300792 | Mouse GPR81-R203, aa 193–230 | + | + | § | § |
Sigma-Aldrich, SAB1300791 | Mouse GPR81-C7, aa 7–36 | + | + | − | − |
Novus Biologicals, NLS2095 | 19 aa peptide from C-terminus of human HCAR1 | + | + | § | § |
Novus Biologicals, NBP1–51956 | C-QQLARQARMKKATR (internal region) | + | + | § | § |