. 2019 Jun 5;39(23):4422–4433. doi: 10.1523/JNEUROSCI.2092-18.2019

Table 1.

Anti-HCAR1 primary antibody specificity

Anti-HCAR1 primary antibody (source, reference)	Epitope	IHC		WB
Anti-HCAR1 primary antibody (source, reference)	Epitope	WT	HCAR1 KO	WT	HCAR1 KO
Santa Cruz Biotechnology, SC-32647	C-terminal, extracellular domain of human HCAR1	§	§	+	+
Santa Cruz Biotechnology, SC-32648	C-terminal, cytoplasmic domain of human HCAR1	+	+	−	−
Sigma-Aldrich, SAB1300090	Mouse GPR81-S296, aa 276–329	+	+	+	+
Sigma-Aldrich, SAB1300793	Rat GPR81-R320, aa 286–332	+	+	§	§
Sigma-Aldrich, SAB1300089	Human GPR81–296, aa 310–353	+	+	+	+
Sigma-Aldrich, SAB1300792	Mouse GPR81-R203, aa 193–230	+	+	§	§
Sigma-Aldrich, SAB1300791	Mouse GPR81-C7, aa 7–36	+	+	−	−
Novus Biologicals, NLS2095	19 aa peptide from C-terminus of human HCAR1	+	+	§	§
Novus Biologicals, NBP1–51956	C-QQLARQARMKKATR (internal region)	+	+	§	§

Experiments were performed in brain slices of cortex, hippocampus, and cerebellum. +, positive signal/band; −, negative/absence of signal/band; IHC, immunohistochemistry; WB, Western blot; §, not tested. See Table 1-1, and Table 1-2.