Table 4.

HCAR1 interactions with GABA_B and α_2A receptors and neuronal activity

	WT		HCAR1 KO
	Frequency (%)	ncells, nexp	Frequency (%)	ncells, nexp
GABABR/HCAR1
3,5-DHBAa	32.3 ± 6.9	37, 4
→ + baclofen	2.6 ± 3.3*
Baclofen	13.9 ± 0.8	12, 3	53.2 ± 3.4	35, 4
→ + 3,5-DHBA	43.6 ± 5.5*		51.7 ± 4.5 n.s.
α2AR/HCAR1
3,5-DHBA	48.4 ± 8.9	44, 4
→ + Guanfacine	8.4 ± 3.3*
Guanfacine	24.2 ± 2.8	36, 4	26.8 ± 8.4	29, 4
→ + 3,5-DHBA	54.2 ± 4.1*		27.2 ± 8.2n.s.

Data are expressed as the percentage of the frequency observed in baseline conditions and as the mean ± SEM. n.s., not significant ANOVA, Bonferroni correction, for n cells from n experiments. Statistical significance is indicated for comparisons between the two displayed conditions.

^aConcentrations used: 1 mm 3,5-DHBA, 0.5 μm baclofen, and 10 μm guanfacine.

*p < 0.05.