Table 6.

Mechanism of HCAR1 interaction with GABA_B and α_2A receptors

	Frequency (%)	ncells, nexp
GABABR/HCAR1
Baclofena	41.5 ± 7.6	13, 3
→ + 3,5-DHBA + gallein	41.3 ± 6.96n.s.
Baclofen	36.6 ± 16.2	19, 3
→ + 3,5-DHBA + U73122	12.5 ± 2.4n.s.
α2AR/HCAR1
Guanfacine	40.5 ± 6.0	22, 4
→ + 3,5-DHBA + gallein	46.0 ± 6.3n.s.
Guanfacine	51.2 ± 11.7	17, 3
→ + 3,5-DHBA + U73122	47.5 ± 9.5n.s.

Data are expressed as a percentage of the frequency observed in baseline conditions or as the mean ± SEM. n.s., not significant. ANOVA, Bonferroni correction, for n cells from n experiments. Statistical significance indicated for comparisons between the two displayed conditions.

^aConcentrations used: 1 mm 3,5-DHBA, 0.5 μm baclofen, 10 μm guanfacine, 10 μm gallein, and 10 μm U73122.