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sEH is mainly expressed in astrocytes in the mPFC. A, Western blot analysis of sEH in the brain of Ephx2−/− and control littermates. B, Brain slices (40 μm) were prepared from adult Fgfr3-tdTomato mice and stained with anti-sEH (green) and anti-GFAP (magenta). Scale bar, 20 μm. C, The statistical analysis indicates that >80% of tdTomato-positive cells in the mPFC colocalized with GFAP and most GFAP-positive cells were tdTomato-positive cells in the mPFC.
