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. 2019 Jun 5;39(23):4606–4623. doi: 10.1523/JNEUROSCI.3069-18.2019

Figure 6.

Figure 6.

Generation of Fgfr3-Ephx2−/− mice. A, Schematic diagram of Ephx2loxp/loxp allele generation: the conditional Ephx2 allele has loxp sites flanking exon 2 and generates an out-of-frame mutation after Cre-mediated recombination. B, PCR genotyping of Ephx2loxp/loxp mice. C, Representative confocal images showing coexpression of sEH (green) in GFAP-positive astrocytes (red) in the mPFC of adult Fgfr3-Ephx2−/− mice and littermate controls. Scale bar, 20 μm. D, E, Western blot analysis of the levels of sEH, GFAP, and NeuN in the PFC, hippocampus (Hipp.), cerebral cortex (Cort.), and striatum of adult Fgfr3-Ephx2−/− mice and littermate controls. The levels of sEH were decreased in the PFC, hippocampus, cerebral cortex, and striatum by 66%, 58%, 57%, and 72%, respectively. F, Representative images of adult Fgfr3-Ephx2−/− and littermate control animals. G, Gross appearance of the brain of Fgfr3-Ephx2−/− and littermate control mice. H, H&E-stained coronal sections around the PFC (top) and hippocampus (bottom) of the brains of Fgfr3-Ephx2−/− and littermate control mice. Scale bar, 500 μm. I, Immunofluorescence for GFAP (green) in the hippocampus and NeuN (green) in the mPFC of Fgfr3-Ephx2−/− and littermate control mice. Scale bar, 20 μm. **p < 0.01, ***p < 0.001.