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. 2019 Jun 5;39(23):4606–4623. doi: 10.1523/JNEUROSCI.3069-18.2019

Figure 9.

Figure 9.

Impaired EET signaling in the mPFC induces a depressive-like phenotype. A, mPFC infusion of 14,15-EEZE increased the duration of immobility in the FST at the optimal concentrations. B, mPFC infusions of 14,15-EEZE had no effect on locomotion. C, D, Western blots and quantification of sEH monomer and oligomer in the mPFC of mice injected with pLenti-EGFP (control), pLenti-hEPHX2 (hEPHX2), or pLenti-Lys55Arg (Lys55Arg). E, F, Immobility of adult C57BL/6J mice injected with pLenti-hEPHX2, pLenti-Lys55Arg, or control virus in the FST. G, Schematic of the overexpression of hEPHX2 or control EGFP followed by subthreshold defeat (top), and behavioral analyses were conducted before (Pre-defeat) and after (Post-defeat) subthreshold defeat (bottom). H, Schematic of TAM-induced Ephx2 deletion in astrocytes, followed by overexpression of hEPHX2 or control EGFP in the mPFC in the FST. I, J, Immobility time (I) and total distance (J) of Fgfr3-Ephx2−/− mice and littermate controls injected with pLenti-hEPHX2 or control virus. K, Schematic of Ephx2 deletion followed by hEPHX2 overexpression in the mPFC in the CSDS paradigm (top), and social interaction measured before and after CSDS (bottom). Data are mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.