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. Author manuscript; available in PMC: 2019 Jun 7.
Published in final edited form as: Anal Chem. 2019 Mar 13;91(7):4656–4664. doi: 10.1021/acs.analchem.9b00021

Figure 7.

Figure 7.

(A) Integrated spectra of unstained (autofluorescence) SUP-B15 cells or cells stained with anti-TdT-FITC or 7-AAD. Fit curves (color lines) are overlaid on data (black lines). (B) Multiplexed unmixing of SUP-B15 cells stained with anti-TdT-FITC only, 7-AAD only (model B-cells), or both markers (model CLCs). Data without signal was expanded near the axis for illustration purposes only. (C) Box plots of Sint and SNR for 7-AAD and anti-TdT-FITC staining. Statistical analysis showed p < 10−4 (**).