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Variables Tested for TDI-SFC to Improve TDI Synchronization and Fluorescence Sensitivity
| TDI-SFC variable |
range | improvement | figure |
|---|---|---|---|
| cell focusing | none → 1D focusing | synchronization:4.5 × Speak,0.8× Δt | 3 |
| cell velocity | 1.41 → 1.83 ← 2.11 mm/s | synchronization: 2.3 × Speak, 0.5× Δt | 4 |
| excitation power | 4 → 21 mW | sensitivity: >9× Sint | 5A |
| objective NA | 0.5 → 0.75 NA | sensitivity: 8–10× Sint, 4× SNR | 5B,C |
| TDI-integration time | 200 → 400 ms | sensitivity: 2× Sint, 1 × SNR | 5D |
