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. 2019 Apr 29;11(6):e9856. doi: 10.15252/emmm.201809856

Figure 5. Nf1/NF1‐mutant autochthonous LUAD tumors are sensitive to glutaminase and aminotransferase inhibition in vivo .

Figure 5

  • A
    Schematic describing Kras LSL‐G12D/+; p53 fl/fl (KP) mice intratracheally infected with pSECC lentiviruses containing sgNf1.3 or control sgTom. Mouse tumor burden was tracked by micro‐computed tomography (micro‐CT) post‐infection, and treatment was initiated on week 8 post‐infection. Lungs were finally harvested on week 21 post‐infection.
  • B, C
    Quantification by micro‐CT of mean tumor volumes in (B) CB‐839‐, (C) AOA‐, or vehicle‐treated KP mice derived from 50 randomly selected tumors at 8, 12, 16, and 20 weeks post‐infection with control sgTom or sgNf1.3. Vehicle‐treated KP mice are the same control group for (B) and (C) (n = 21 mice each).
  • D, E
    Quantification by micro‐CT of total tumor volume per mouse in (D) CB‐839‐, (E) AOA‐, or vehicle‐treated KP mice after infection with control sgTom or sgNf1.3 at 8, 12, 16, and 20 weeks post‐infection. Vehicle‐treated KP mice are the same control group for (D) and (E) (n = 21 mice each).
  • F, G
    Relative responses of KP mice tumors treated with (F) CB‐839, (G) AOA, or vehicle starting from week 8 post‐infection and measured until week 20 (n = 21 mice each). Relative response = average tumor volume with treatment/average tumor volume with vehicle.
  • H
    Depictions and quantitation of total tumor burden (total tumor area/total lung area) in CB‐839‐, AOA‐, or vehicle‐treated KP mice after infection with control sgTom or sgNf1.3 at 20 weeks post‐infection. Vehicle‐treated KP mice are the same control group in both boxplots (n = 21 mice each).
Data information: P‐values are reported in Appendix Table S3. In bar charts and line graphs, data presented as means with error bars representing standard deviations (SDs). For boxplots, whiskers indicate the minimum and maximum values, the upper and lower perimeters represent the first and third quartiles, the midline represents the median value, and the x symbol represents the mean.