Figure 2. . Microscopic tissue sample stained in the flourescence in situ hybridization method.

Biopsy tissue from herniated disc tissue retrieved during surgery. Biofilm (green) covered aggregates of Propionibacterium acnes bacteria. The close presence of pylomorphonuclear leucocytes (lilac) indicate inflammatory immune response.

Reproduced with permission from John Wiley/Rightslink (2018) [35].