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. Author manuscript; available in PMC: 2019 Jun 7.
Published in final edited form as: Cell Rep. 2019 May 7;27(6):1755–1768.e4. doi: 10.1016/j.celrep.2019.04.049

Figure 4. Endothelial TLR4 Is Critical for Sensing LPS-Induced Responses in Decidua.

Figure 4.

(A and B) Relative expression of Il6 (A) and Il10 (B) mRNAs in Tlr4f/f and Tie2Cre/+Tlr4f/f mice after a low dose (2.5 μg/mouse) or high dose (40 μg/mouse) of LPS treatment for 1 hour. n = 4. *p < 0.05. Results are shown as mean ± SEM.

(C) Ratio of mRNA levels of Il10 versus Il6 in deciduae of Tlr4f/f mice under a low and high dose of LPS. n = 4. *p < 0.05. Results are shown as mean ± SEM.

(D) western blotting of protein levels for pStat3 in Tlr4f/f and Tie2Cre/+Tlr4f/f mice after 1 h of a low or high dose of LPS treatment on day 16 of pregnancy, n = 4.

(E) Co-immunostaining of pStat3 (red) and CD31 (green) in LPS-treated deciduae of Tlr4f/f and Tie2Cre/+Tlr4f/f mice. Scale bar of upper panels, 500 μm. Bottom panels show images of higher magnification of those within the demarcated rectangles (Scale bar, 100 μm). Arrowheads indicate endothelial cells. Dec, deciduae; Pl, placenta.

(F) pStat3 nuclear translocation by IL-6. Isolated stromal cells were treated with recombinant IL-6 (50 ng) for 30 min. Scale bar, 50 μm.

(G and H) pStat3 levels (G) and Il10 mRNA levels (H) after recombinant interleukin 6 (rIL-6) challenge. Tubulin and rpL7 were used as internal controls for western blotting and qPCR, respectively. n = 3. *p < 0.05. Results are shown as mean ± SEM.

See also Figures S5, S6, and S7.