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. Author manuscript; available in PMC: 2019 Jun 7.
Published in final edited form as: Anal Chem. 2016 Nov 16;88(23):11821–11829. doi: 10.1021/acs.analchem.6b03499

Figure 3.

Figure 3.

Single-point measurements of DrBphP-PCM photoswitching. (A) Pulse sequence used for photoswitching/excitation of a single droplet/single cell: τ643 = 13 ms, dark relaxation time τdark (τdark = Δcycle − 3Δ643 − 2τ643 − Δ783 − τ). (B, C) Measurements with a single droplet of 30 μM DrBphP-PCM solution: (B) 783 nm laser is off, photoswitching does not occur, slow decrease in amplitude indicates bleaching by 643 nm laser; and(C) 783 nm laser is on, different amplitudes of pulses show that stable photoswitching occurs within 250 cycles. (D, E) Measurements of the protein photoswitching in a single cell at different pulse durations/energies.