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. Author manuscript; available in PMC: 2019 Jun 7.
Published in final edited form as: Anal Chem. 2016 Nov 16;88(23):11821–11829. doi: 10.1021/acs.analchem.6b03499

Figure 4.

Figure 4.

In-flow photoswitching of DrBphP-PCM protein: (A) amplitudes of fluorescent pulses from DrBphP-PCM droplets when 783 nm laser is off and photoswitching does not occur; (B) amplitudes of fluorescent pulses from DrBphP-PCM droplets when the 783 nm laser is on and photoswitching occurs; (C, D) dependence of switching contrast on intensity of the 643 nm laser and speed of droplets for 30 μM and 15 μM DrBphP-PCM protein solutions; and (E) dependence of photoswitching contrast on intensity of the 783 nm laser for 15 μM DrBphP-PCM protein solution.