Fig. 5.

Results of tri-alanine substitution mutation analysis. Tri-alanine mutants were created in Twinkle and Pol1A to test for interaction disruption. Mutated residues were selected based off of DCA analysis results. While only two mutations caused a disruption of Twinkle with Pol1A, most mutations weakened or completely disrupted interaction with Pol1B. Mutations in Pol1A were much more distinct, either completely disrupting interaction with Twinkle, or failing to affect this interaction at all