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. 2019 Jun 6;19:241. doi: 10.1186/s12870-019-1854-3

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Truncations of Pol1A and Pol1B interacting with residues 120–295 of Twinkle. Truncations are numbered 1–12 and span Pol1A and Pol1B as designated above. Each truncation was tested for association with the interacting region of Twinkle and plated in 1:5 spot dilutions on selective and non-selective media as a control. Non-selective media is SD -leu -trp and the selective media is SD -leu -trp -his -ade. Note the stronger interaction between Pol1A and Twinkle versus Pol1B and Twinkle