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. 2019 Jun 6;19:540. doi: 10.1186/s12885-019-5725-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — E7-specific CD8 T cells appearance in TC-1 mice vaccinated with adjuvanted E7 SLP. On day 0, mice (n = 20 per group) were implanted s.c. (footpad) with 2e4 TC-1 cells and then vaccinated s.c. (base of tail) on day 14 with 3 μg E7 SLP + CpG in w/o emulsion or adjuvant alone. Three mice from each group were sacrificed at 5 time points between days 14–28 and spleen and tumor tissue analyzed by FACS for E7-dextramer⁺ CD8 T cells within a total CD8⁺ population (spleen) and b total CD45⁺ population (tumor). c Tumor tissue from an additional mouse per group was immunohistochemically stained for the presence of CD8 T cells. All images 40X. Scale bars = 25 μm