Fig. 3.

The percentage of CD3⁺, CD3⁺CD4⁺, and CD3⁺CD8⁺ T lymphocytes in spleen. The spleen was separated from the mice at 35 day. a The representative flow cytometry plots showing CD3⁺, CD3⁺CD4⁺, and CD3⁺CD8⁺ T lymphocytes in the spleen. The quantification of the frequencies were shown in b CD3⁺, c CD3⁺CD4⁺ and d CD3⁺CD8⁺ T cells. Data were expressed as the mean ± SD. * P < 0.05, ** P < 0.01