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The proliferation of lymphocytes in spleen. Splenocytes were prepared at 35 day and cultured with PBS, Con A, B. subtilis-PRV, B. subtilis-gCa and B. subtilis-gDa for 72 h at 37 °C. Then, the proliferative responses detected by CCK8 method and shown as a proliferation ratio. Data were expressed as the mean ± SD. **P < 0.01
