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. 2019 May 31;13:53. doi: 10.3389/fnana.2019.00053

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Copyright © 2019 Dillingham, Mathiasen, Frost, Lambert, Bubb, Jankowski, Aggleton and O’Mara.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A,B) Schematic representations of crystallin mu (Crym) delineated boundaries of the claustrum anterior to the striatum (CPu), highlighting the density of ectopic Crym-positive cell soma within the ventral (vCLA; black) and dorsal claustrum (dCLA; red). (C) shows representative Crym staining in the claustrum/insular while (D) shows a pixel density heat map of (C) highlighting 1. The difficulty associated with determining the boundary between vCLA and dCLA; and 2. Cortical fibers crossing the claustrum to join the internal capsule. (E–H) show equivalent panels from striatal levels in which the number of ectopic Crym-positive cell soma is reduced and the boundary between vCLA and dCLA is more distinct. ec, external capsule; fm, forceps minor of the corpus callosum. Scale bars = 200 μm.