FIGURE 5.

Crystallin mu (Crym) is a cortical marker that is expressed particularly strongly in the insular cortex. In contrast, expression in the claustrum is considerably reduced providing contrast for delineation of the anatomical boundary of the claustrum. Pixel density plots through the insular cortex at both striatal levels (C; red), and rostral to the striatum (A,B; black and blue, respectively) show cortical peaks either side of a claustral trough (asterisks). Central schematic diagram shows approximate coronal levels of photomicrographs in (A,B). (D–F): Schematic representations of Crym-based delineation of the claustrum-endopiriform complex. Delineation of the dorsal endopiriform nucleus (DEn) is not possible using Crym, however, vCLA, and to a lesser degree dCLA are (See also Figure 3). acc, nucleus accumbens; CPu, caudate/putamen; ec, external capsule; fm, forceps minor of the corpus callosum; Ins, insular cortex; LO, lateral orbital cortex. Scale bars in A,B = 300 μm; Aⁱ,Bⁱ = 200 μm; C = 600 μm.