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. 2019 May 31;13:53. doi: 10.3389/fnana.2019.00053

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Copyright © 2019 Dillingham, Mathiasen, Frost, Lambert, Bubb, Jankowski, Aggleton and O’Mara.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fiber label resulting from multiple injections (bilateral) of an anterograde viral tracer [AAV-CaMKIIa-hM4D(Gi)-mCherry] in the anterior cingulate cortex (case 219#3; column 2) shown in sections co-labeled for parvalbumin (column 1). The four rows (A–D) show photomicrographs of the claustrum at four anteroposterior levels separated by 200 μm. Row (D) is at the most rostral portion of striatum, Rows (C–A) rostral to striatum. Terminal fiber label co-localized with parvalbumin (PV) in a plexus that extends at least two sections rostral to striatum. The dotted line indicates the border between lateral orbital and insular cortices. Ins, insular cortex; LO, lateral orbital cortex. “AAV-mCherry” is an abbreviation for AAV-CaMKIIa-hM4D(Gi)-mCherry. Scale bars = 200 μm.