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. 2019 Jun 7;15:188. doi: 10.1186/s12917-019-1941-6

Fig. 2.

Fig. 2

Immunogenicity of EVs from different mycobacterial strains. Isolated EVs from M. bovis (a), MAP (b) and Mtb H37Rv (c) were separated by SDS-PAGE and submitted to immunoblot with sera from two different cows grouped according to the following treatments: (1) (PTB- TB-), naïve cows; (2) (PTB+) Paratuberculosis positive; (3) (TB+) Bovine tuberculosis positive and; (4) (PTB- Vacc) PTB- and MAP vaccinated cows. d Mtb EVs were also submitted to immunoblot against Mtb EVs-specific serum. The asterisk indicates the presence of the 19 kDa antigen