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. 2019 Jun 7;15:188. doi: 10.1186/s12917-019-1941-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Immunogenicity of EVs from different mycobacterial strains. Isolated EVs from M. bovis (a), MAP (b) and Mtb H37Rv (c) were separated by SDS-PAGE and submitted to immunoblot with sera from two different cows grouped according to the following treatments: (1) (PTB- TB-), naïve cows; (2) (PTB+) Paratuberculosis positive; (3) (TB+) Bovine tuberculosis positive and; (4) (PTB- Vacc) PTB- and MAP vaccinated cows. d Mtb EVs were also submitted to immunoblot against Mtb EVs-specific serum. The asterisk indicates the presence of the 19 kDa antigen