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. 2019 Jun 7;15:188. doi: 10.1186/s12917-019-1941-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Reactivity of bovine sera to M. tuberculosis EV-associated lipoprotein LpqH. a Purified LpqH was separated by SDS-PAGE and submitted to immunoblot with sera from seventeen different cows grouped according to the following treatments: (1) (PTB- TB-), naïve cows; (2) (PTB+) Paratuberculosis positive; (3) (TB+) Bovine tuberculosis positive and; (4) (PTB- Vacc) PTB- and MAP vaccinated cows. Positive reactivity and absence of reactivity are indicated on top of each panel as “+” or “-”, respectively. b Inverse titers of LpqH-specific IgG antibodies measured by ELISA in the same seventeen bovine sera as in (a). Data are mean +/− sem. The results are representative of two independent and similar experiments. (**** P < 0.0001 one-way ANOVA with Tukey post-test)